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. 2008 May 21;3(5):e2197. doi: 10.1371/journal.pone.0002197

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Colomb et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Flat preparations are shown of stage 15 eagle-Gal4, UAS-mcD8-GFP embryos, labeled with a Cy3-conjugated anti-HRP antibody to visualize the VNC architecture (red) upper panels; and with a polyclonal anti-GFP antibody, secondarily detected by Cy2-anti rabbit (green) lower panels, with the merged images in the middle. To help the lecture of the phenotypes, a diagram is provided. Only one segment is shown, but the corresponding entire cords are provided on Figure S1. eagle-positive neuronal behavior is shown A in a wild-type background, B in the transheterozygous (Df en^X31/Df gsb^X62) background (arrowhead indicates neuronal progeny of the NB 6-4, and arrow indicates neuronal progeny of the NB 7-3), C and D in the transheterozygous (Df en^X31/Df gsb^X62) background, when restoring GsbN expression in eagle-positive cells. Note that when commissures appear thicker, in 56% of the segments, two types of results are obtained and correspond to the pictures provided in C and D.