Skip to main content
. 2008 May;10(5):450–461. doi: 10.1593/neo.08120

Figure 2.

Figure 2

Endogenous wild-type p53 interacts with accumulated exogenous mutant p53. Immunoprecipitations were conducted with a non-specific antibody, or an anti-V5 antibody that recognizes the exogenous mutant p53 protein. Western blots of the immunoprecipitates were conducted with an anti-p53 clone DO-1 antibody that recognizes both mutant and wild-type p53. (A) One representative Western blot from three independently immunoprecipitated samples. Lane 1 is 30 µg of whole cell lysate (WCL) from HME1 as a positive control for p53. Even lanes 2 to 14 are immunoprecipitates of the nonspecific antibody control. Odd lanes 3 to 15 are V5 antibody-specific immunoprecipitates. (B) Levels of wild-type p53 relative to the mutants in each HME1 mutant p53-expressing cell line. The quantification and standard error of the mean were calculated from Western blots of three independent experiments and show the amounts of wild-type p53 (w.t.) relative to the mutant p53 (mut) in each of the HME1 mutant p53-expressing cell lines (R175H, R273H, R280K, and R249S).