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Purification of VP1-Z. Escherichia coli strain BL21(DE3) that contained the plasmid pVP1Z was treated as described. Along the purification scheme, aliquots were taken and analyzed by 12% SDS-PAGE. VP1-Z is marked by an arrow. (1) Molecular weight standard; (2) soluble cell lysate; (3) VP1-Z after S-Sepharose; (4) VP1-Z pentamers after gel filtration; (5) VLPs of VP1-Z after gel filtration.
