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Stability of VLPs of VP1-Z and wtVP1 VP1-Z and wtVP1 at a protein concentration of 20 μg/mL in assembly buffer without ammonium sulfate, respectively, were incubated at a heating rate of 0.3°C/min from 5°C to 80°C. The change in fluorescence during heating was monitored using 295 nm for excitation and 340 nm for emission. The transition midpoint of denaturation of VP1-Z VLPs (open circle) and wtVP1 VLPs (filled circle) was determined with 51°C and 54°C, respectively.
