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Mutations in the monomer–monomer interface of LacI. This structure depicts one of the two dimers of the IPTG-bound structure of LacI (1lbh; Lewis et al. 1996). The N-terminal DNA binding domain is not resolved for this structure. Mutations affecting interface strength and assembly (at positions 84, 98, 74, 282, and 354) are indicated with spheres. D278, which forms an ion pair with H74, is indicated with the smaller balls and sticks.
