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Local mutants. This structure depicts the core C-subdomain interface for one of the two dimers of the DNA-bound structure of LacI (1efa; Bell and Lewis 2000). Positions of mutations that revert the phenotype of Y282D (223, 246, 248, 274, 276, and 284) are indicated with spheres. D278, which does not form an ion pair with H74 in this conformation, is indicated with the smaller balls and sticks.
