Fig. 6.

Neutrophil chemotaxis is attenuated following oxidative stress by treatment of HUVEC with UEA-II. Oxidative stress significantly increased neutrophil chemotaxis compared to normoxic cells. Treatment of HUVEC with UEA-II (100 nmol/L) significantly attenuated neutrophil chemotaxis following endothelial oxidative stress. Neutrophil chemotaxis to HUVEC following oxidative stress was measured by analysis of myeloperoxidase levels and transformed to neutrophil count with a standard curve (n = 3, error bars = SEM, *P < 0.05 compared to normoxia, P < 0.05 compared to vehicle-treated hypoxia/reoxygenation cells).