. 2001 Apr;10(4):715–724. doi: 10.1110/ps.38101

Table 1.

Thermodynamic parameters derived from DSC measurements of denaturation of P1 variants of BPTI determined in 10 mM glycine-HCl buffers

P₁	pH	T_den (°C)	ΔH_cal (kcal • mole⁻¹)	ΔH_vH (kcal • mole⁻¹)	ΔH_cal/ΔH_vH	ΔC_p,den (kcal • K⁻¹•mole⁻¹	Concn. (μg/mL)	ΔG_den^b (kcal • mole⁻¹)
Trp	2.0	75.6	65.12	65.84	0.99	0.22	200	−1.03
	2.5	78.8	66.00	69.00	0.99	0.43	120	−0.85
Ile	2.0	76.5	65.12	67.61	0.96	0.36	140	−0.85
	2.5	79.8	67.49	69.28	0.97	0.37	250	−0.65
	3.0	85.6	68.54	70.23	0.97	0.43	120	−0.69
Phe	2.0	77.2	65.24	67.61	0.96	0.24	140	−0.72
	2.5	80.2	66.24	67.61	0.98	0.55	90	−0.56
	3.0	85.8	68.80	74.30	0.93	0.43	150	−0.69
Tyr	2.0	77.3	65.90	64.32	1.02	0.40	300	−0.70
	2.5	80.5	67.20	66.20	1.02	0.36	130	−0.49
	3.0	85.1	70.06	69.30	1.01	0.40	120	−0.78
Leu	2.0	77.7	66.40	63.30	1.05	0.33	80	−0.63
	2.5	81.7	67.90	69.00	0.98	0.25	50	−0.28
	3.0	87.0	69.52	73.10	0.95	0.27	60	−0.43
Val	2.0	77.9	66.91	68.56	0.97	0.29	180	−0.60
	2.5	80.3	68.20	70.71	0.96	0.57	120	−0.57
	3.0	85.4	70.02	73.58	0.95	0.41	140	−0.77
Met	2.0	78.8	66.00	63.80	1.03	0.40	130	−0.42
	2.5	80.0	67.90	70.70	0.96	0.31	60	−0.63
	3.0	86.8	70.00	69.90	1.00	0.32	90	−0.45
Glu	2.0	77.2	71.70	79.10	0.94	0.20	250	−0.36
Gln	2.0	79.5	66.50	65.20	1.02	0.32	200	−0.28
	2.5	82.8	68.10	68.70	0.99	0.23	230	−0.06
	3.0	88.2	70.20	67.00	1.05	0.36	250	−0.18
Ala	2.0	79.6	67.30	66.30	1.02	0.42	100	−0.27
Thr	2.0	80.8	67.50	70.40	0.96	0.24	200	−0.04
	2.5	84.4	68.70	66.00	1.04	0.30	220	0.23
	3.0	90.6	73.20	73.30	1.00	0.26	120	0.29
Ser	2.0	81.4	68.49	69.71	0.98	0.45	210	0.08
	2.5	84.6	68.99	70.00	0.98	0.43	120	0.29
	3.0	90.4	72.15	76.45	0.94	0.41	120	0.27
Gly	2.0	81.5	67.75	68.25	0.99	0.24	260	0.09
	2.5	84.7	68.25	68.56	0.99	0.29	280	0.30
	3.0	89.8	70.95	72.15	0.98	0.19	220	0.13
Asp	2.0	82.0	67.85	70.71	0.96	0.33	230	0.19
	2.5	84.4	70.20	76.00	0.92	0.28	200	0.27
	3.0	89.4	71.20	76.00	0.94	0.36	120	0.06
Asn	2.0	82.3	67.99	70.47	0.96	0.36	200	0.25
	2.5	84.9	69.52	72.38	0.96	0.43	200	0.36
Lys^a	2.0	83.3	68.00	63.00	1.08	0.22	110	0.44
	2.5	86.3	70.00	67.80	1.03	0.3	90	0.60
	3.0	91.7	71.00	69.80	1.02	0.35	120	0.49
Arg	2.0	83.6	68.44	67.85	1.00	0.31	270	0.45
	2.5	87.3	70.47	73.58	0.96	0.41	250	0.85
	3.0	93.3	72.29	76.21	0.95	0.33	300	0.87
His	2.0	86.7	69.90	69.28	1.00	0.33	180	1.09
	2.5	90.4	71.74	74.30	0.96	0.29	150	1.48
	3.0	95.5	73.27	75.49	0.97	0.33	180	1.30

^a Recombinant Lys15, Met52Leu BPTI.

^b Free energy change of unfolding were calculated at 81°C, 83°C, and 89°C (the mean T_den values of P₁ variants at pH 2.0, 2.5, and 3.0, respectively).