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MALDI TOF of the DOPA analogs of neurotensin N6 (A) and proctolin (B) cross-linked with tyrosinase. The peptides were incubated at a 50:1 peptide to enzyme weight ratio in a 10-mM HEPES pH 7.5 with stirring at room temperature. These spectra correspond to a 30-min incubation period. Parenthetical numbers correspond to the mass loss as a result of cross-linking and oxidation of one or more DOPA functionalities in the multimers.
