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Ultraviolet (UV)-visible spectra of DOPA-neurotensin N6 incubated with tyrosinase (A) and periodate (B). In both cases, the peptide was incubated in a 10-mM HEPES pH 7.5 buffer at room temperature. Tyrosinase was added at a 50:1 peptide to enzyme ratio. The scans correspond to 0, 5, 15, 30, 60, and 120 min. Periodate was added at different molar ratios to the DOPA content. Scans correspond to 0:1, 1:1, 10:1, 20:1, 30:1, and 50:1 periodate:DOPA molar ratios.
