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SDS-PAGE (12.5%) of TMPKmt. Lane 1, the protein molecular weight standards (arrows) were the 10 kDa Protein Ladder from Gibco BRL. The arrowhead corresponds to the 50 kDa band; lane 2, bacterial extract (20 μg of proteins); lane 3, pool after Blue Sepharose chromatography (20 μg of proteins); lane 4, enzyme after Ultrogel AcA54 chromatography (10 μg of proteins).
