Table 4.
Kinetic parameters of TMPKmt with various nucleoside monophosphates
| NMP | Kmapp (mM) | Vm (μmole/min • mg of protein) | Ki (mM) |
| dTMP | 0.04 | 10.60 | |
| 3′F-TMP | 0.03 | 0.11 | |
| dehydro-TMP | 0.14 | 0.16 | |
| dUMP | 2.10 | 3.50 | |
| 5Br-dUMP | 0.03 | 9.82 | |
| 5I-dUMP | 0.14 | 7.46 | |
| 5F-dUMP | 0.42 | 4.66 | |
| 5-methyl isodCMP | 0.13 | ||
| AZT-MP | 0.02 |
The reaction medium (0.5 mL final volume) was the same as described in Table 2. The concentration of ATP was kept constant at 0.5 mM whereas the concentration of nucleoside monophosphates was varied within 0.05 and 3.6 mM.