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Kinetic parameters of the purified mutant Asn233Ala IMP-1 metallo-β-lactamase at pH 7.5 and 30°C
| Antibiotics | kcat (s−1) | Km (μM) | kcat/Km (μM−1 s−1) |
| Ampicillin | 230 ± 20 | 110 ± 18 | 2.1 |
| Nitrocefin | 1488 ± 488 | 13 ± 5 | 118 |
| Cefotaxime | 495 ± 40 | 30 ± 4 | 16.3 |
| Cephaloridine | 654 ± 141 | 148 ± 32 | 4.4 |
