Table 3.

Secondary structure content estimated from far UV CD spectra of rRBP and rRBP mutants

Protein	Temperature (°C)	pH	% α-Helix	% β-Sheet	Random	Quality of fit
rRBP (structure)			9	45	47	—
rRBP	20	7.4	8	45	47	pa
rRBP	20	2.0	24	40	36	g
rRBP	80	7.4	8	41	51	g
rRBP24Y	20	7.4	14	34	52	p
rRBP22A/24F	20	7.4	14	34	52	p
rRBP91H	20	7.4	9	47	44	p
rRBP67L/91H	20	7.4	8	44	48	p
rRBP105F	20	7.4	11	40	49	p
rRBP67L/91H/105F	20	7.4	15	35	51	p

^a (p) a poor fit of the calculated spectrum to experimentally determined spectrum (maximum error > 0.227); (g) a good fit (maximum error < 0.227).

The analyses were conducted using the k2d program (Andrade et al. 1993).