Table 1.

Background values for different components used in phage preparations

Component	Concentration	I (kHz)
SB medium	undiluted	557.8
Ampicillin	100 mg/mL	73.7
Tetracycline	5 mg/mL	40.4
IPTG	800 mM	5.7
PEG NaCl	20%/15%	2.6
PBS buffer	undiluted	1.1
1× PEG precipitated phage*	122 nM	69.9
2× PEG precipitated phage*	1.4 nM	8.4
CsCl purified phage*	66 nM	9.1
RhoGr anti-M13 labeled phage	170 nM	81.6

I is the mean fluorescence intensity. *The phage were resuspended in PBS buffer after treatment. The background does not depend on the concentration of phages but on phage medium components.