Table 3.

Binding and displacement of the Cy5-labelled antigen rE2 to E2 specific phage

Sample	Conc (nM)	τ 1 (ms)	%	τ 2 (ms)	%	τ 3 (ms)	%	N
Free Cy5	0.82	0.0652	100	—	—	—	—	0.252
Cy5-streptavidin	3.95	0.0652	51	0.319	49	—	—	0.997
E2—Cy5	2.14	0.0652	34.4	0.319	0	0.548	65	0.539
phage + E2Cy5	2.89	0.0652	47.7	0.548	37.5	1.8	14.8	0.730
displacement	3.52	0.0652	58	0.548	36.5	1.8	5.5	0.889

τ represents the different diffusion times (measured in ms), % (percentage) is the relative fraction of each component in the reaction and N gives the average number of molecules in the volume element. The concentration in this table is calculated from the N values and gives total number of labeled molecules.