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Cysteine site-directed mutagenesis
| Wild-type | Mutant | |||
| AA | Codon | AA | Codon | Antisense oligonucleotidesa |
| Ser-71 | AGT | Cys-71 | TGC | 5′287TTGAGGTGAGCACC[GCA]TATGTGACCCACA2583′ |
| Gly-72 | GGT | Cys-72 | TGT | 5′287TTGAGGTGAGC[ACA]GCTGATGTGACCC2613′ |
| Ala-73 | GCT | Cys-73 | TGT | 5′287TTGAGGTG[ACA]ACCGCTGATGTGAC2633′ |
| Cys-189 | TGT | Ser-189 | TCC | 5′643GGGCAGGGTTGATTCC[GGA]GCCAGTGTAG6153′ |
a The underlined bases are mismatched; bases in brackets represent codons of mutants.
