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. 2001 Aug;10(8):1677–1684. doi: 10.1110/ps.49901

Fig. 2.

Fig. 2.

Multiple sequence alignment of Haspin-related proteins and Cdc2 (CECDC2A) from C. elegans. The alignment was generated and is colored and annotated as described for Figure 1 ▶. Above the alignment is a secondary structure prediction using PHDsec (Rost and Sander 1993), based on the alignment without CECDC2A. Only regions I through VII of the kinase domain are shown because many of the hypothetical translations of the C. elegans genome lack homology with kinase regions VIII and XI, probably because of incorrect prediction of C-terminal exons by gene prediction algorithms. The alignment includes residues 563–810 of C01H6.9 (GenBank CAA95786), 71–309 of F22H10.5 (GenBank AAB09102), 37–238 of ZK177.2 (GenBank AAG00045), 169–377 of Y48B6A.10 (GenBank CAB54446), 395–592 of H12I13.1 (GenBank AAF39881), 71–281 of C04G2.10 (GenBank CAA94675), 309–525 of Y73B6A.1 (GenBank AAF36040), 144–352 of T05E8.2 (GenBank AAB52426), and residues 1–176 of C. elegans CECDC2A (GenBank AAC60520, CAA48455). The PSI-BLAST E values after a single iteration when searching with human Haspin residues 461–798 (to allow comparison with Fig. 1 ▶) were C01H6.9, 10−103; F22H10.5, 2 × 10−97; ZK177.2, 10−21; Y48B6A.10, 3 × 10−21; H12I13.1, 2 × 10−25; C04G2.10, 2 × 10−30; Y73B6A.1, 6 × 10−63; and T05E8.2, 2 × 10−48. More severely truncated Haspin-like kinase domains not shown were also identified in C. elegans: F12A10.2, F12A10.6, F59E12.6 (all closely related to ZK177.2), C06E4.5, C26E6.1, C50H2.7, and K08B4.5 (GenBank accession numbers AAA68295, AAA68299, AAB54256, AAA82472, AAA21155, CAA98255, and AAC68982).