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Nanospray spectra of 11 μM IclR-8 protein (the truncated protein) in 0.5 M NH4HCO3, 1 mM DTT. (A) At 100 V declustering voltage, showing two ion envelopes, the 20–24+ ions of tetramer, and the 14–17+ ions of dimer. There is little evidence of fragmentation or of monomer ions. (B) At 200 V declustering voltage, showing three ion envelopes, with highly charged monomer ions produced at the expense of the others.
