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Nanospray spectrum for 13 μM GclR protein in 0.5 M NH4HCO3, 1 mM DTT. At 120V declustering voltage, showing two ion envelopes, the 23–28+ ions of tetramer, and the 16–20+ ions of dimer. As with the IclR protein under gentle ionization conditions, there is little evidence of either monomer formation or fragmentation.
