Fig. 6.

Nanospray spectrum of 11 μM IclR-8 protein plus 5 μM consensus DNA in 10 mM NH₄HCO₃, 1 mM DTT, at 140 V declustering voltage. This spectrum was acquired over 40 min (compare to identical conditions used for the complex in Fig. 5 ▶). The major ion species represent a tetramer plus one dsDNA. The deconvolution (inset) gives mass 134,380 daltons at poor resolution (450 daltons FWHM). There is also evidence of uncomplexed protein tetramer, dimer, and monomer.