Table 2.
Stability of hYap wild-type and WW domain variants to GdnHCl denaturation
| WW sequence | ΔGu (H2O) (4°C) (kcal/mol) | ΔΔGu (H2O) (4°C) (kcal/mol) | m (4°C) (kcal/mol/M) |
| Wild type | 0.71 ± 0.10a | — | 1.12 ± 0.03 |
| L30Y | 1.29 ± 0.30a | 0.58 | 1.12 ± 0.11 |
| D34T | 1.15 ± 0.08a | 0.44 | 1.09 ± 0.02 |
| A20R | 1.58 ± 0.04 | 0.87 | 1.04 ± 0.02 |
| A20R/D34T | 1.88 ± 0.04 | 1.17 | 0.96 ± 0.01 |
| A20R/L30Y | 2.47 ± 0.04 | 1.76 | 0.97 ± 0.01 |
| A2R/L30Y/D34T | 3.17 ± 0.07 | 2.46 | 0.92 ± 0.02 |
a Higher error is expected for data fits with little or no pretransition baseline (Fig. 7C ▶).