Fig. 1.

Complete hydrolysis of dUTP by dUTPase under multiple-turnover conditions. The inset shows the linear transformation of the data between the arrows, according to the integrated Michaelis–Menten equation adapted for enzymes with product inhibition as described in Materials and Methods, and the corresponding regression line. The reaction was recorded at 573 nm after reacting 30 nm dUTPase and 25 μM dUTP in the presence of 25 mM MgCl₂, 2500 μM BICINE, and 50 μM cresol red at pH 8.