Table 2.
Michaelis-Menten, specificity and inhibition constants of viral (EIAV, HSV-1 and MMTW), bacterial ( E. coli) and Leishmania major dUTPases towards different nucleoside triphosphates and uracil triphosphate analogues
| Substrate/inhibitor | L. major | E. colia | EIAVb | HSV-lc | MMTVd |
| dUTP: Km(kcat/Km) | 2.11 (2.3 × 107) | 0.2 (4 × 107) | 1.1 (2 × 107) | 0.3 (2 × 107) | 0.8 (2 × 106) |
| dTTP:Km(kcat/Km) | 1514 (5.5 × 103) | > 20000 (34) | 260 (< 2000) | 400 (1000) | N.D. (2000) |
| dCTP:Km(kcat/Km) | > 2500 (5.0 × 103) | 4000 (< 100) | 3000 (1000) | 1000 (2000) | N.D. |
| UTP:Km(kcat/Km) | > 2500 (< 2 × 103) | 2500 (< 100) | N.D. | 1000 (200) | N.D. |
| dUDP:Km(kcat/Km) | 62.70 (1 × 106) | Ki = 15 | Ki = 3.6 | Ki = 17 | N.D. |
| dUMP: Kip | 13.05 | 1500 | 130 | 170 | N.D. |
| α-β-imido-dUTP: Ki | 0.89 | 5 | 0.6 | 0.9 | N.D. |
The units for Km and Kiare μM and for kcat/Km M−1s−1. All values were obtained at pH 8.
a Larsson et al. 1996b.
b Nord et al. 1997.
c Bergman et al. 1995.
d Bjornberg and Nyman 1996.