Figure 3.

(a) Effects of pertussis toxin (PTX) and cholera toxin (CTX) on monocyte migration in response to 400 ng ml⁻¹ Tat_1–86, 1 μg⋅ml⁻¹ CysL_24–51, and 100 ng⋅ml⁻¹ MCP-3 and f-MLP. PTX caused the marked inhibition of chemotaxis to all the chemoattractants tested, CTX did not substantially affect migration. (b) Effects of PTX and CTX on calcium mobilization induced by 10 nM MCP-1, 20 nM Tat_1–86, or 100 nM CysL_24–51 peptide in monocytes.