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Dependence of the packing parameter (f, defined in Eq. 1) on the radial region over which the atom summation is made. Results of calculations are given for spin-labeled cysteine mutants: A25C (circles), V31C (squares), and A49C (triangles), either with (solid symbols) or without (open symbols) hydrogens included in the evaluation. The radius of the region was measured from the reference atom, which was the nitrogen atom of the proxyl ring of the spin label.
