Table 2.

Orientation of the spin-label N-O bond to the axis of the transmembrane helix, for structure number 20 of the M13 coat protein (PDB: 2CPS) optimized with single cysteine-maleimide substitutions at different positions, in the absence and presence of the lipid shell

Spin-labeled residue no.	Without lipid	With lipida
25	67°	51°
31	104°	111°
36	107°	95°
38	100°	97°
46	77°	40°b
49	70°	70°

^a Final structure optimized with +1 Å vertical shift of the protein.

^b Adjusted manually, producing an isoenergetic structure (see text).