Table 1.

Aggregation properties of monellin (MN) chains at high temperature ^a

	Aggregation		Dye binding
	Turbidity	Shape	ThT	CR
MN, no heating	−	−	−	−
no cosolvent	−	−	−	−
0.3 M NaCl	+	fibrous	+	+
0.5 M urea	+	irregularc	±	−
A1 chain,b 0.3 M NaCl	−	−	−	−
A2 chain,b 0.3 M NaCl	−	−	−	−
B chain, 0.3 M NaCl	+	fibrous	+	+
SMN, 0.3 M NaCl	+	irregular	±	−

ThT, thioflavin T; CR, congo red; SMN, a recombinant single-chain monellin.

^a 270 μM of MN or SMN and 200 μM of the separate subunit chains were incubated at 67.5°C for 2 h, and then recovered at 25°C for 1 h.

^b A1 or A2 chain is the A chain of MN without or with an additional Phe residue in the N-terminal part, respectively.

^c The irregular aggregate contained some fibrous and granular components (Fig. 3B ▶).