Fig. 2.
(A) Deviations of the CαH chemical shifts of desG10(1–16) (black bar) and U(1–17) (clear bar) from literature random coil values. (B) Deviations of the CαH chemical shifts of desG10(1–16) (black bar) and U(1–17) (clear bar) from the chemical shifts of U(1–7) and U(1–17) for the sequence 1–7 and 11–17 and literature random coil values for residues 8, 9, and 10. The sequence of U(1–17) was adopted in the x-axis. The two α-protons of Gly-10 in U(1–17) are shown separately. There is no Gly-10 in desG10(1–16). ΔΔ > 0 for downfield shifts. The pH of the solutions were 3.8 for U(1–17), U(1–7), and U(11–17), and 1.9 for desG10(1–16) to take advantage of improved solubility at this pH. The chemical shifts observed are independent of pH over the pH of interest in this study.