Fig. 8.

Saturation transfer assignment of the Thr-12 and Thr-14 resonances in denatured ubiquitin. (A) 1D spectrum of ubiquitin in the vicinity of the midpoint of denaturation in the presence of 3.1 M GdnDCl, pH 3 at 320 K. (B) Difference spectrum obtained by subtraction from A of a comparable spectrum acquired with presaturation of the peak originating from the >-methyl protons of native Thr-12 and Thr-14 (1.085 ppm). Individual assignments of Thr-12 and Thr-14 were made by analogy with the U(1–17) and U(1–34) peptides.