Far-UV–CD spectra of native wild-type CspA (filled circles), Y42W (plus signs), S52W (open squares), and T68W (open triangles). The protein concentration was approximately 50 μM, and the buffer contained 50 mM potassium phosphate, 100 mM KCl at pH 7.0. The spectra were recorded at 25°C in a 1-mm cuvette.