Fig. 1.

Western blot analysis of rCRFR1 and rCRFR1-Kif and binding of rUcn to rCRFR1, rCRFR1-Kif, and rCRFR1-NT-Kif. Membrane preparations with a total protein content of 11 μg, which were obtained from HEK 293 cells producing either rCRFR1 (A) or rCRFR1-Kif (B) were applied. The absence or presence of PNGaseF is indicated. (C) Competitive binding was performed using [¹²⁵I-Tyr⁰]-rUcn as radioligand and increasing concentrations (10 pM–3.16 μM) of rUcn. Data represent duplicates from two independent experiments. Binding curves were normalized by total binding in absence of competitor [B₀].