Fig. 6.

Disulfide bridge arrangement of rCRFR1-NT-Kif. (A) and (B) show the disulfide-linked peptides of the tryptic and AspN digest, respectively. (C) represents the derived disulfide bridging. In the amino acid sequences, X represents an asparagine or aspartate residue depending on the extent of glycosylation at the corresponding position. The solid lines represent disulfide bridges directly deduced from the proteolytic digests. The dotted lines connect fragments without an unambiguous assignment of a single disulfide bridge. The dashed line represents the disulfide bridge which was concluded from the results of Cys derivatization. The 23-amino acid long signal peptide is marked by a black background and the 24 amino acid long signal peptide by a gray background.