Table 1.
Binding properties of rCRFR1, rCRFR1-Kif, and rCRFR1-NT-Kif
| Peptide (radioligand used) | rCRFR1 KD or KI [nM] | rCRFR1-Kif KD or KI [nM] | rCRFR1-NT-Kif KD or KI [nM] |
| rUcn ([125I-Tyr0]-rUcn) | 0.61 ± 0.05 (n = 3) | 0.79 ± 0.17 (n = 3) | 27 ± 10 (n = 4) |
| h/rCRF ([125I-Tyr0]-h/rCRF) | 1.0 ± 0.2 (n = 3) | 1.2 ± 0.2 (n = 2) | no binding |
| Ast ([125I-Tyr0]-rUcn) | 4.9 ± 1.0 (n = 3) | 3.4 ± 0.4 (n = 3) | 60 ± 24 (n = 3) |
The affinity constants are mean ± sdv of n independent binding experiments performed in duplicate. KD values were calculated for rUcn and h/rCRF and KI values for Ast.