Table 2.

Stimulation of intracellular cAMP accumulation by h/rCRF and rUcn in HEK 293 cells producing rCRFR1 or rCRFR1-Kif

Peptide	rCRFR1		rCRFR1-Kif
	EC50 [nM]	cAMP/cells	EC50 [nM]	cAMP/cells
h/rCRF	0.33 ± 0.06	90 ± 7 pmol/104	0.27 ± 0.04	83 ± 9 pmol/104
rUcn	0.89 ± 0.10	94 ± 3 pmol/104	0.50 ± 0.09	78 ± 14 pmol/104

The values are mean ± sdv of at least four independent determinations performed in duplicate.