Fig. 2.

Analysis of products for synthesis directed by a mRNA template. The reaction products were separated on a 2.0% (w/v) agarose gel and visualized on a Molecular Dynamics Phosphorimager plate. The size markers are 50 bp and 1 kb-labeled DNA ladders. The column labeled "C" is the control reaction without enzyme. For the L435K mutant and RT24, the columns from left to right indicate decreasing concentration of enzyme, with each enzyme at 40 nm, 13.3 nm, and 4.4 nm. A similar distribution of full-length products, 600 and 650 bases long, can be seen for both enzymes.