Figure 1.
SDS–PAGE of fractions eluted from the HisTrap Fast Flow column. Lane M, molecular-weight markers (kDa); lanes 1–9, HisTrap elution fractions at 0.144, 0.153, 0.250, 0.259, 0.269, 0.279, 0.288, 0.298 and 0.307 M imidazole, respectively.
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