Figure 1.
Three-dimensional structure of recombinant HEV-VLPORF3/ORF2 at a resolution of 24 Å determined by cryo-EM and image reconstruction. An isosurface representation of the outer surface of recombinant HEV-VLPORF3/ORF2 is shown viewed along the icosahedral twofold axis. The surface density was contoured at a level corresponding to 100% mass of the expected particle volume. The particle is color-coded to differentiate two distinct domains: the shell domain (white) and the protrusion domain (yellow). The surface capsid conforms to T = 1 icosahedral symmetry in which the 60 subunits are arranged into 30 protruding spikes with the homodimers as the basic building blocks at each icosahedral twofold axis. The scale bar represents 100 Å.