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. 2008 Mar 29;64(Pt 4):318–322. doi: 10.1107/S1744309108007197

Figure 2.

Figure 2

Purity and integrity of recombinant HEV-VLPORF3/ORF2 crystals grown in 4%(w/v) PEG 8000, 100 mM Tris–HCl pH 8.5 analyzed by SDS–PAGE (a) and negative-stained electron microscopy (b). In (a), the left lane contains molecular-weight markers (kDa) and the right lane contains the protein band of recombinant HEV-VLP from a dissolved crystal. In (b), a recombinant HEV-VLPORF3/ORF2 crystal was crushed with a nylon loop and stained with 2% uranyl acetate; the VLPs remained with intact capsid morphology after dissolving from the crystal. The bar represents 1000 Å.