Figure 3.

Analysis of Nm-Csp by size-exclusion chromatography with and without single-stranded DNA (107 µg). Overlaid size-exclusion chromatograms (each using 200 µg of protein) showing the monomeric Nm-Csp (orange trace) eluting at 14.8 ml and the Nm-Csp–dT₆ heterodimer eluting at 13.6 ml (green trace). The elution volumes of the calibrants along with their molecular weights are represented by vertical dotted lines. The A ₂₅₄/A ₂₈₀ ratios for the eluted peaks were 2.94 for the protein alone and 9.91 for the protein plus oligonucleotide.