Figure 3.

Effects of anti-p150^Glued antibodies on the dynein–dynactin interaction. Optic lobe cytosol was loaded onto p150^Glued affinity columns that were preadsorbed with either preimmune serum or UP235 serum. The columns were eluted with 0.5 M NaCl in motility buffer. The resulting load, flowthrough, wash, and eluate fractions were analyzed by SDS/PAGE stained for total protein with Coomassie blue (A) and by immunoblotting (B) using antibodies to the dynein heavy chain (DHC, a generous gift of David Asai) or the dynein intermediate chain (DIC, a generous gift of Walter Steffen).