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EL-4-CAR cells transduced with an adenoviral FasL effectively kill FasR-expressing target cells. EL-4-CAR 13 cells were transduced (MOI of 100) with Ad-FasL, AdCMV-GFP, or no virus (control). The cells then were washed once and coincubated for 20 hr with 51Cr-labeled L1210-FasR cells at a 5:1 or 0.5:1 effector/target ratio. Each value represents the mean of triplicate measurements ± SD.
