Table 2.
Effect of CD47/IAP transfection on the electrophysiological response of CD47-null cells to mechanical stimulation
| Membrane potential (-mV) (mean ± SEM) | ||||
| Frequency of stimulation | Cell (phenotype) | Resting | Post mechanical stimulation | Percentage change |
| 0.33 Hz | 3657L (CD47 wild-type+) | 10.9 ± 0.27 | 4.5 ± 0.64 | -59a |
| 3656 1/Sra (CD47-null) | 14.0 ± 1.36 | 13.8 ± 0.46 | -1b | |
| 3656 1/171 (mouse CD47 type 2+) | 13.1 ± 0.52 | 5.9 ± 0.65 | -55a | |
| 3656 1/315 (human CD47 type 2+) | 11.8 ± 1.03 | 4.6 ± 1.03 | -61a | |
| 0.083 Hz | 3657L (CD47 wild-type+) | 10.8 ± 0.6 | 11.9 ± 0.72 | +10b |
| 3656 1/Sra (CD47-null) | 12.4 ± 0.43 | 13.0 ± 0.27 | +5b | |
| 3656 1/171 (mouse CD47 type 2+) | 12.8 ± 0.53 | 20.4 ± 0.92 | +48a | |
| 3656 1/315 (human CD47 type 2+) | 12.2 ± 0.91 | 17.2 ± 0.32 | +42a | |
| OV10 58A2 (CD47-null) | 10.2 ± 0.85 | 10.8 ± 1.50 | +6b | |
| OV10 315 (human CD47 type 2+) | 7.0 ± 0.84 | 18.6 ± 1.86 | +165a | |
| OV10 164/315 (β3/CD47 type 2+) | 35.4 ± 1.6 | 49.2 ± 3.34 | +39a | |
| OV10 148A10T (CD47IgV/GPI) | 22.6 ± 1.33 | 22.2 ± 1.07 | 0b | |
CD47-null cells or cells transfected with either type 2 CD47 cDNA, CD47 extracellular immunoglobulin variable (IgV) domain linked to a glycosylphosphatidylinositol anchor, or vector control were studied. Membrane potentials of cells were measured at rest and after 20 minutes of mechanical stimulation at 0.33 or 0.083 Hz. N = 5. ap ≤ 0.01; bnot significant; SEM, standard error of the mean.