Table 1.

Primers used for semi-quantitative PCR and product length

Gene encoding	Primer sequence	Size (base pairs)	Cycles	Tm (°C)
L27	Sense: 5'-TCCTGGCTGGACGCTACTC-3'	225	27	62
	Antisense: 5'-CCACAGAGTACCTTGTGGGC-3'
MCP-1	Sense: 5'-ATGCAGTTAATGCCCCACTC-3'	167	29	57
	Antisense: 5'-TTCCTTATTGGGGTCAGCAC-3'
bFGF	Sense: 5'-GAACCGGTACCTGGCTATGA-3'	182	31	61
	Antisense: 5'-CCGTTTTGGATCCGAGTTTA-3'
VEGF	Sense: 5'-CAATGATGAAGCCCTGGAGT-3'	211	32	64
	Antisense: 5'-TTTCTTGCGCTTTCGTTTTT-3'
TNF-α	Sense: 5'-AGATGTGGAACTGGCAGAGG-3'	178	31	58
	Antisense: 5'-CCCATTTGGGAACTTCTCCT-3'
IL-1β	Sense: 5'-TGAAAGCTCTCCACCTCAATGG-3'	366	28	61
	Antisense: 5'-TCCATGGTGAAGTCAACTATGTCC-3'

MCP-1, monocyte chemotactic protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; T_m, melting temperature.