Figure 3.

Effect of incubation temperature on the development of distilled sulfur mustard (HD) and distilled lewisite (L) toxicity in human skin keratinocytes. Proliferating cultures of first passage neonatal human skin keratinocytes were treated with either HD (A) or L (B), and then incubated at 25°C, 31°C, or 37°C. At 1, 2, 3, or 4 days after treatment, unique test cultures were assayed for viability using alamarBlue. The HD-treated cultures assayed at 4 days, were refed, and then incubated for an additional 24 hours at 37°C before viability assessment. Data represent the mean ± SEM of three different experiments using tissue from three different donors, and was analyzed by analysis of variance and post hoc Dunnett's Method Comparison testing. *Significantly different from the median lethal concentrationobtained at 24 hours at 37°C, P < .05.