Figure 3.
Monocyte reactive oxygen species (ROS) generation in patients across the continuum of sepsis. ROS generation was measured by 2',7'dichlorofluorescein (DCFH) metabolism. Monocytes were gated based on FSC versus SSC parameters and the expression of CD14 on cell surface. ROS generation was analyzed in histograms and expressed as the geometric mean fluorescence intensity (GMFI). *p < 0.01 compared to healthy volunteers; #p < 0.05 compared to the severe sepsis group.