Figure 3.

Fibrinogen binds specifically to cardiomyocyte ICAM-1. Colocalization of fibrinogen and cardiomyocyte intracellular adhesion molecule-1 (ICAM-1). (a) Isolated cardiomyocytes were incubated with Oregon Green-labeled fibrinogen (green) and fluorescently stained for ICAM-1 (red). A multiphoton dual-excitation image of the contour of the cell of interest is shown. By means of an overlay of images, strong colocalization of fibrinogen and ICAM-1 was indicated by a yellow color. (b) A representative image of a rat cardiomyocyte with adherent fibrinogen-coated polystyrene beads (white arrows) is shown to the left of the graph. The specificity of the ICAM-1-fibrinogen interaction is demonstrated as anti-ICAM-1 antibody pre-treatment results in significantly less fibrinogen-coated polystyrene beads adherent to the cardiomyocytes (*p < 0.05 versus control). Ab, antibody; CL, control; IgG, immunoglobulin G.