Figure 4.

ICAM-1 (8–22) binds to fibrinogen. Two-step enzyme-linked immunosorbent assay in which fibrinogen was immobilized on the 96-well plate and then incubated with biotinylated intracellular adhesion molecule-1 (ICAM-1) (8–22) peptide or biotinylated 'scrambled' control peptide. Anti-biotin antibody was added, and colorimetric absorbance quantified the peptide-fibrinogen interaction. (a) Representative ICAM-1 (8–22) dose-response curve showing absorbance plateau at a fibrinogen concentration of approximately 100 μM. (b) Group mean absorbance data taken at the plateau fibrinogen concentration (100 μM) demonstrating a strong interaction between the ICAM-1 (8–22) peptide and fibrinogen compared with a small non-specific interaction between the scrambled peptide and fibrinogen. *p < 0.05 versus scrambled peptide.