Table 3.

Tilled genes and mutation density in Caméor mutant population

Tilled genes	Amplicon size (bp)	% of GC in exons	Identified mutants	Screened M2 families	Mutation frequency
Ps CONSTANS-like a (PsCOLa)	1,012	46.30%	11	1,536	1/141 Kb
LectineA	971	40.80%	13	1,536	1/115 Kb
Sucrose transporter (SUT1)	1,014	52.40%	12	1,536	1/130 Kb
Cell wall invertase (cwINV)	1,612	41.50%	12	1,536	1/206 Kb
Serine-threonine proteine kinase (Sym29)	2,457	44.00%	8	768	1/236 Kb
Phosphoenolpyruvate carboxylase (PepC)	1,009	44.40%	25	3,072	1/124 Kb
Lec1-like (L1L)	870	39.10%	21	4,608	1/191 Kb
DOF transcription factor 2 (PsDOF2)	1,200	36.60%	9	3,072	1/410 Kb
Trypsine inhibitor (TI1)	712	34.20%	13	3,840	1/210 Kb
Pea albumine (PA2)	746	38.50%	9	3,072	1/255 Kb
Anther specific protein (End1)	851	40.50%	31	3,072	1/84 Kb
MADS box gene (PM10)	1,302	34.60%	20	4,608	1/300 Kb
MADS box gene (PM2)	1,390	31.30%	28	4,608	1/229 Kb
Tendril-less transcription factor (TL)	1,104	34.00%	28	3,072	1/121 Kb
Eukaryotic translation initiation factor (eiF4e)	1,383	36.90%	36	4,608	1/177 Kb
Eukaryotic translation initiation factor (eIF(iso)4e)	772	36.70%	10	4,608	1/356 Kb
Methyl transferase 1 (Met1)	3,842	40.20%	96	4,704	1/188 Kb
Retinoblastoma related (RBR)	2,959	40.80%	72	4,608	1/112 Kb
Late embryogenesis abundant protein (PsLEAM)	952	44.00%	17	4,608	1/258 Kb
Heat shock protein 22 (HSP22)	622	45.66%	18	4,608	1/159 Kb
Total/mean	26,780	40.12%	467	-	1/200 Kb

Part or all of the Caméor mutant population was screened for mutations in the genes listed. The size of the screened amplicon, the number of mutants identified and the mutation frequency for each amplicon are indicated. The average mutation frequency was estimated to one mutation per 200 kb and is calculated as in Greene et al. [6], except that we have summed the sizes of all the amplicons and we divided by the total number of identified mutants.