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. 2008 Feb 26;9(2):R44. doi: 10.1186/gb-2008-9-2-r44

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Copyright © 2008 Rines et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cell cycle validation of isolated genes. (a) Quantitative comparison of mitotic index values based on phospho-histone H3 (pHis) detection in HeLa and U2OS cell lines. (b) Proliferation analysis. Cell counts after 48 hours of small interfering RNA (siRNA) incubation are determined and proliferation is reported as the ratio of cell counts compared with those from the negative control. (c) mRNA knockdown levels were determined using quantitative polymerase chain reaction and are reported as a percentage of target mRNA when cells are transfected with a negative control siRNA. (d) Cell cycle analysis of HeLa cells treated with siRNAs to determine relative G1, S, and G2/M cells per candidate gene validation using flowcytometry.